High Performance Reversed-Phase Columns for the Separation of Small Molecules
The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.
Ultrapure silica with high-density bonding for low silanol activity
Very high efficiencies for maximum resolution
Reproducible manufacturing practices for reproducible column-to-column performance
LC/MS compatible
Acclaim 120 C18 columns have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and available in 3 and 5 μm particles sizes, and , , and mm-diameters, with an average pore diameter of 120 Å. Acclaim 120 C18 is also available in µm RSLC format (see Acclaim Rapid Separation LC columns section for more details). All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.
Wide Range of Applications
The Acclaim 120 C18 can be used for the many diverse reversed-phase C18 separations. Its rugged, reproducible, and reliable characteristics make it appropriate for pharmaceutical, environmental, food and other industrial chromatographic separations. The Acclaim Catalog has many examples of the use of this column.
Acclaim 120 HPLC column production begins with ultrapure silica, which results in symmetrical peak shapes due to ultra-low metal content. Next, a unique bonding chemistry produces a very well-ordered and high-density silica surface coverage, which results in highly predictable reversed-phase separations, unaffected by secondary retention mechanisms.
| Acclaim 120 Silica-Based Reversed-Phase Columns for Small Molecule Analysis Specifications | |
|---|---|
| Particle shape | Spherical |
| Particle size distribution (40/90) | |
| Total carbon content | C18: 18% C8: 1% |
| Carbon surface coverage | C18: μmol/m2 C8: μmol/m2 |
| Pore volume | mL/g |
| Average pore diameter | 120 Å |
| Surface area | 300 m2/g |
| pH Range | 2–8 |
| Temperature | <60 °c |
| End-capped | Yes |
| Metal impurity (Na, Fe, Al) | <>< ppm individual metal |
| USP listing | C18: L1 C8: L7 |
| Starting material | Ultrapure silica |
| Particle size | 3, 5 µ |
Standard particle sizes are 3 and 5 μm. Analytical columns are available in , , and mm diameters; standard lengths are 50, 100, and 150 mm. Capillary formats are available in 75 and 300 μm, mm, and custom diameters, and in 50, 150, or 250 mm lengths. Micro precolumn and nano precolumn cartridges are available in several formats. Guard columns in both and mm sizes packed with 5 μm particles are recommended to protect analytical columns. Columns for fast LC are listed in the RSLC section.
| Guard Columns | |
|---|---|
| Acclaim 120, C18, 5µm Guard Cartridges, ( x 10mm) 2 ea; requires holder 069580 | 069689 |
| Acclaim 120 C18, 5µm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580 | 071981 |
| Acclaim 120, C18, 5 µm Guard Cartridges, ( x 10 mm), (use V-2 holder) | 069695 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| Analytical Columns | |
|---|---|
| Acclaim 120, C18, 3 µm Analytical ( x 50 mm) | 059128 |
| Acclaim 120, C18, 3 µm Analytical ( x 100 mm) | 059129 |
| Acclaim 120, C18, 3 µm Analytical ( x 150 mm) | 059130 |
| Acclaim 120, C18, 3µm Analytical ( x 250mm) | 076187 |
| Acclaim 120, C18, 3µm Analytical ( x 100mm) | 076186 |
| Acclaim 120, C18, 3 µm Analyitcal ( x 150 mm) | 063691 |
| Acclaim 120,C18, 3µm, Analytical, ( x 250mm) | 070077 |
| Acclaim 120, C18, 3 µm Analytical ( x 50 mm) | 059131 |
| Acclaim 120, C18, 3 µm Analytical ( x 100 mm) | 059132 |
| Acclaim 120, C18, 3 µm Analytical ( x 150 mm) | 059133 |
| Acclaim 120, C18, 5 µm Analytical ( x 50 mm) | 059142 |
| Acclaim 120, C18, 5 µm Analytical ( x 100 mm) | 059143 |
| Acclaim 120, C18, 5 µm Analytical ( x 150 mm) | 059144 |
| Acclaim 120, C18, 5 µm Analytical ( x 250 mm) | 059145 |
| Acclaim 120, C18, 5 µm Analytical ( x 50 mm) | 059146 |
| Acclaim 120, C18, 5 µm Analytical ( x 100 mm) | 059147 |
| Acclaim 120, C18, 5 µm Analytical ( x 250 mm) | 059149 |
| Acclaim 120, C18, 5 µm Analytical ( x 150 mm) | 059148 |
| Acclaim RSLC 120 C18 Analytical Columns | |
|---|---|
| Acclaim RSLC 120, C18, 3 µm Analytical ( x 33 mm) | 066272 |
| Acclaim 120 C18, 3µm Analytical, ( x 50mm) | 068971 |
| Acclaim RSLC 120, C18, 3 µm Analytical ( x 75 mm) | 066273 |
| Acclaim 120, C18, 3 µm Analyitcal ( x 150 mm) | 063691 |
| Acclaim RSLC 120, C18, µm Analytical ( x 30 mm) | 071400 |
| Acclaim RSLC 120, C18, µm Analytical ( x 50 mm) | 068981 |
| Acclaim RSLC 120, C18, µm Analytical ( x 100 mm) | 068982 |
| Acclaim RSLC 120, C18, µm Analytical, ( x 150mm) | 071399 |
| Acclaim RSLC 120, C18, µm Analytical ( x 250mm) | 074812 |
| Acclaim RSLC 120, C18, µm Analytical, ( x 30mm) | 071606 |
| Acclaim RSLC 120, C18, µm Analytical, ( x 50mm) | 071605 |
| Acclaim RSLC 120, C18, µm Analytical, ( x 100mm) | 071604 |
| Micro and Nano Columns | |
|---|---|
| Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm × 5 cm, nanoViper | 164567 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm × 15 cm, nanoViper | 164568 |
| 300 µm x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper | 164716 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm × 15 cm, nanoViper | 164571 |
| mm x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper | 164717 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 1 mm × 15 cm, nanoViper | 164572 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm × 5 cm, nanoViper | 164567 |
| 75 µm x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164708 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm x 25 cm | 160326 |
| 300 µm x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper | 164716 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm × 15 cm, nanoViper | 164571 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm x 25 cm | 160300 |
| mm x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper | 164717 |
| mm x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å, nanoViper | 164711 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, mm x 25 cm | 160287 |
| Micro and Nano Precolumns | |
|---|---|
| Acclaim 120, C18, 5 µm, 120 Å, 300 µm x 5 mm, 5 Cartridges | 162326 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, 500 µm x 5 mm, 5 Cartridges | 160446 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, mm x 5 mm, 5 Cartridges | 160434 |
| Acclaim PepMap100, C18, 5 µm, 100 Å, mm x 15 mm, 5 Cartridges | 160438 |
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